Always keep all buffer bottles tightly closed when not actively in use. Thermo scientific genejet plasmid midiprep kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid dna from recombinant e. The genejet plasmid miniprep kit is a simple, rapid, and costeffective system for the isolation of high quality plasmid dna from recombinant e. Norgens plasmid miniprep kit is designed for the rapid preparation of plasmid. Product information thermo scientific genejet plasmid. The readytouse plasmid dna is of high quality in lowsalt tris buffer and suitable for typical downstream applications. C plasmid preparations with the genelute kit, comparing either the standard miniprep procedure or. Ensure that buffers have been prepared according to the instructions on page 3. The following procedure is performed at room temperature. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna from bacterial cultures. Thermo scientific genejet plasmid miniprep kit fisher. Solution ii must be tightly capped when not in use. The kit utilizes silicabased membrane technology in the form of convenient spin column. The system provides a rapid method to purify plasmid dna using a silica membrane column.
Supplier page from thermo fisher scientific for genejet plasmid miniprep kit 250 prep product specs. It is based on binding of dna to silicabased membranes in a chaotropic salt. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Plasmid miniprep kit this kit is designed for the rapid preparation of plasmid dna from small cultures of escherichia coli using convenient spin columns.
Each column has a binding capacity of at least 20 g. Wash the endonucleases from the sample according to the following protocol. Plasmid can be transferred between same species or between different species. The plasmid dna yield will vary depending on the plasmid copy number, type and size, the bacterial strain, the growth conditions, and the kit. The kit passes qc requirements if the purified plasmid dna has an a 260 a. Thermo scientific genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column.
The kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. The plasmid miniprep kit is based on the alkaline lysis of bacterial cells, followed by the binding of the. This miniprep plasmid kit was designed for plasmid dna purification from 17 ml of cultured bacterial cells. By combining silicabinding technology and the convenience of a spin column format, up to 15. The kit recovers up to 20 g of high copy plasmid dna per isolation procedure. The kit utilizes an exclusive silica based membrane technology in the form of a convenient spin column. These are available as convenient pdf files online at. The plasmid dna is preferentially purified from other cellular components such as genomic dna and rna. It is used in recombinant dna experiments to clone genes from other organisms and make large quantities of their dna. Qiaprep spin miniprep kit highyield protocol english pdf. Easypreptm plasmid miniprep 5 6 kit contents at 5,000 g for 10 min or 3,500 rpm for 20 min in a tabletop pellet by vortexing or pipetting. Verify the success of a plasmid dna miniprep using gel electrophoresis.
C plasmid preparations with the genelute kit, comparing either the standard miniprep procedure or the miraprep using 1x volume of ethanol. The pureyield plasmid miniprep system isolates highquality plasmid dna for use in eukaryotic transfection and in vitro expression experiments. All centrifugation steps should be carried out at 16,000 x g,000 rpm. If you want to perform plasmid purification without using a kit, you can find a protocol for kitfree plasmid mini prep at the bottom of this page. General description the genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. Reproducible yields of molecular biology grade plasmid dna single protocol for high and lowcopy vectors the apexprep spin miniprep kit is designed for isolation of up to 20.
List the function of each solution used in a plasmid dna miniprep. Place the tubes in a centrifuge balanced and spin at maximum speed for 5 minutes. The pureyield plasmid miniprep system is designed to purify 1. Cloning, sequencing, pcr, transformation and restriction analysis. In increasing order, these are the miniprep, midiprep, maxiprep, megaprep, and gigaprep. Pureyield plasmid miniprep system miniprep kit plasmid isolation. Genejet plasmid miniprep kit from fermentas biocompare. Genejet plasmid miniprep kit 250 prep k0503 from thermo. Thermo scientific genejet plasmid miniprep kit thermo fisher. One 1 l of eluted plasmid dna was visualized post agarose gel. The entire miniprep procedure can be completed in 30 minutes or less, depending on the number of samples processed.
The method employs a modification of the alkaline method of cell lysis1 and a microspin cup with a silicabased fiber matrix that binds dna in the presence of a chaotropic. We provide a wide range of size formats from mini to gigapreps as well as options for highthroughput processing. This system can be used to isolate any plasmid from e. A rapid protocol to isolate highquality plasmid dna, using a silica membrane column, in less than 10 minutes, depending on the number of samples. Users of the qiagen miniprep kit may notice a strong similarity between the two kits. To help you choose which kit is the best choice for your application see our plasmid kit selection guide. All other materials should be stored at room temperature. Gencatch tm plasmid dna mini preparation provides a simple, fast and costeffective method to purify plasmid dna without phenolchloroform extraction. Gencatch tm plasmid dna miniprep kit epoch life science. By combining silicabinding technology and the convenience of a spin column format, up to 15 g of high copy plasmid dna can be recovered from. The phenol extraction and ethanol precipitation are not required, and the highquality plasmid dna is eluted in a small volume of tris buffer included in the. Designed for rapid small scale isolation and purification of high copy and low copy plasmid dna.
The kit utilizes silicabased membrane technology in the form of convenient spin columns. L of elution buffer to the column and incubate 2 minutes. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators. Genejet plasmid miniprep kit components of the kit. Let tubes stand on ice for 5 minutes its ok if they go longer. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna.
For processing larger volumes, the presto midi plasmid kit is also available. Thermo scientific genejet plasmid miniprep kit life. The genejet plasmid miniprep kit is designed for rapid and costeffective smallscale preparation of high quality plasmid dna from recombinant li cultures. The protocol below is meant to describe the general procedure for purifying plasmid dna from bacterial cultures. Miniprep plasmid purification kit specifications according to the manufacturers protocol. If you plan to perform transfections and prefer a miniprep format try our nucleospin plasmid transfectiongrade kits. Thermo scientific genejet plasmid miniprep kit for 50. The genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from.
Ensure that rnase a is added to the resuspension solution before the first use. Thermo scientific genejet plasmid midiprep kit is qualified by isolating high copy plasmid dna from 50 ml of overnight e. The objective of this lab is to perform extraction of plasmid dna and analyze the results. Solution i once rnase a is added should be stored at 28. Thus plasmid dna remains in solution while proteins and other dna molecules precipitate.
The geneaid midi plasmid kit uses prepacked anionexchange resin columns to purify plasmid dna from 50300 ml of cultured bacterial cells. The axyprep plasmid miniprep kit is based upon a modified sdsalkaline lysis of bacterial cells in combination with selective binding of the plasmid dna to a special miniprep column. The mirapreps in c included fresh addition of rnase 50. Pureyield plasmid miniprep system protocol promega corporation. Plasmid is a double stranded, circular extra chromosomal dna of bacterium. The quality of isolated dna has been evaluated in a set of tests. Purified plasmid dna is suitable for all routine downstream applications including bacterial cell transformation, mammalian cell transfection, dna sequencing, restriction enzyme digestion, cloning, and pcr. Recovering plasmid dna from bacterial culture addgene. The strataprep plasmid miniprep kit provides a rapid, phenolfree method for purifying plasmid dna from bacterial cultures.
Of the four kits tested, the pureyield plasmid miniprep system. Genejet plasmid miniprep kit k0502, k0503 wash the column add 500. The protocol provides a simple and reliable method to achieve the rapid isolation of highly purified plasmid. The purelink quick plasmid miniprep kit is designed for rapid isolation of sequencinggrade plasmid dna from li in 3045 minutes, using silica membrane columns. The results illustrate the very high binding capacity of the columns in the isolate ii plasmid mini kit. The genejet plasmid maxiprep kit is designed for large scale isolation of high quality plasmid dna from recombinant li cultures. Both plasmid kits include trueblue lysis buffer an optional color indicator to prevent common handling errors while ensuring efficient cell lysis, neutralization and maximum plasmid product yield.
Thermo scientific genejet plasmid miniprep kit has been tested in the isolation of puc19 dna from e. The genejet plasmid miniprep kit is designed for rapid and costeffective smallscale preparation of high quality plasmid dna from recombinant e. The genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. Minipreparation of plasmid dna is a rapid, smallscale isolation of plasmid dna from bacteria. Na0150s genelute hp plasmid miniprep kit 10 preps na0150 genelute hp plasmid miniprep kit 70 preps na0160 genelute hp plasmid miniprep kit 350 preps related products cat. The plasmid miniprep kit is based on the alkaline lysis of bacterial cells, followed by the binding of the dna onto the glass fiber matrix of the spin column in the presence of high salt. Product description pkg size l2542 lb broth 500 ml t9650 tae buffer 105 1 l t4415 tbe buffer 105 1 l g2526 gel loading solution 5 ml. Genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. Fastreadytouse plasmid dna typically in 3045 minutes flexibleformats work with. An average yield of 120 ug plasmid dna can be expected from 15 ml overnight bacterial culture. If you will be using a kit, follow the kits instructions. Features of the purelink quick plasmid miniprep kit.
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